human huvec nucleofector kit Search Results


human stem cell nucleofecto kit  (Lonza)
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Lonza human stem cell nucleofecto kit
Human Stem Cell Nucleofecto Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pasmcs  (Lonza)
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Lonza human pasmcs
Human Pasmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human t cell nucleofector kit  (Lonza)
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Lonza human t cell nucleofector kit
Human T Cell Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human t cell nucleofector kit  (Amaxa)
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Amaxa human t cell nucleofector kit
Human T Cell Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human umbilical vein endothelial cells (huvecs  (Lonza)
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Lonza human umbilical vein endothelial cells (huvecs
Human Umbilical Vein Endothelial Cells (Huvecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human monocyte nucleofector kit  (Lonza)
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Lonza human monocyte nucleofector kit
Human Monocyte Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human coronary artery endothelial cells hcaecs  (Lonza)
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Lonza human coronary artery endothelial cells hcaecs
Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on <t>human</t> <t>coronary</t> artery endothelial cells <t>(HCAECs)</t> treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.
Human Coronary Artery Endothelial Cells Hcaecs, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human aortic smooth muscle cells nucleofection kit  (Amaxa)
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Amaxa human aortic smooth muscle cells nucleofection kit
Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on <t>human</t> <t>coronary</t> artery endothelial cells <t>(HCAECs)</t> treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.
Human Aortic Smooth Muscle Cells Nucleofection Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human stem cell nucleofection buffer  (Lonza)
90
Lonza human stem cell nucleofection buffer
Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on <t>human</t> <t>coronary</t> artery endothelial cells <t>(HCAECs)</t> treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.
Human Stem Cell Nucleofection Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human stem cell nucleofector kit 1  (Amaxa)
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Amaxa human stem cell nucleofector kit 1
Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on <t>human</t> <t>coronary</t> artery endothelial cells <t>(HCAECs)</t> treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.
Human Stem Cell Nucleofector Kit 1, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human intestinal microvascular endothelial cells himecs  (Lonza)
97
Lonza human intestinal microvascular endothelial cells himecs
Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on <t>human</t> <t>coronary</t> artery endothelial cells <t>(HCAECs)</t> treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.
Human Intestinal Microvascular Endothelial Cells Himecs, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human monocyte nucleofector kit  (Amaxa)
90
Amaxa human monocyte nucleofector kit
Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on <t>human</t> <t>coronary</t> artery endothelial cells <t>(HCAECs)</t> treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.
Human Monocyte Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human monocyte nucleofector kit/product/Amaxa
Average 90 stars, based on 1 article reviews
human monocyte nucleofector kit - by Bioz Stars, 2026-03
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Image Search Results


Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on human coronary artery endothelial cells (HCAECs) treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.

Journal: American Journal of Physiology - Cell Physiology

Article Title: Shear stress induces Gα q/11 activation independently of G protein-coupled receptor activation in endothelial cells

doi: 10.1152/ajpcell.00148.2016

Figure Lengend Snippet: Ligand- and shear stress-induced activation of the sphingosine-1-phosphate (S1P3)-Gαq/11 complex. In situ proximity ligation assay (PLA) was performed using antibodies directed against S1P3 and Gαq/11 (A and B), serotonin (5-HT)2A and Gαq/11 (C and D), S1P3 and G protein-coupled receptor kinases (GRK2; E), and S1P3 and β-arrestin-1/2 (F) on human coronary artery endothelial cells (HCAECs) treated with vehicle alone (Control), S1P; 2 μM for 30 s) (A, E, and F), or 5-HT (100 nM for 30 s) (C) and on HCAECs that were either unstimulated (Sham) or subjected to a step change in shear stress (Flow) for the indicated times (B, D, E, and F). Representative confocal images in a single z-plane of cells probed with the S1P3/Gαq/11 antibody pair are shown (A and B). Scale bar, 20 μm. Each bar graph shows quantification of at least 3 independent experiments as PLA signal (red dots) per cell (blue nuclei) relative to the control condition, with error bars indicating SE; n = 6 for C, n = 5 for A, B, E (Flow), and F (Flow), n = 3 for D, E (S1P), and F (S1P). *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Human coronary artery endothelial cells (HCAECs) from male donors were obtained from either Cell Applications (San Diego, CA) or Lonza (Walkersville, MD) and maintained in complete endothelial growth medium (EGM-2; Lonza) supplemented with 10% heat-inactivated FBS and penicillin-streptomycin.

Techniques: Activation Assay, In Situ, Proximity Ligation Assay